May T W, Kaiser M L
J Assoc Off Anal Chem. 1984 May-Jun;67(3):589-93.
A cryogenic grinding technique, which involves brittle fracture of samples by impact pressure, was evaluated for the final reduction of whole-fish samples for subsequent metal determinations. The technique incorporated a number of desirable features: protection of heat-labile components; incorporation of lyophilization to facilitate handling and storage; requirement of few procedural steps; short grinding time per sample; and easy, rapid cleanup of equipment. Particle size in the ground powder was less than 100 microns. Problems associated with the technique were contamination of fish samples with Cr, Fe, Mo, and Ni; limited size of the grinding container; and powder clumping caused by the presence of lipids. Approaches to alleviate these problems are discussed.
一种低温研磨技术，该技术通过冲击压力使样品发生脆性断裂，被评估用于全鱼样品的最终粉碎，以便后续进行金属测定。该技术具有许多理想的特性：保护热不稳定成分；结合冻干以便于处理和储存；所需的程序步骤少；每个样品的研磨时间短；以及设备易于快速清理。研磨后的粉末粒度小于100微米。与该技术相关的问题包括鱼样品被铬、铁、钼和镍污染；研磨容器尺寸有限；以及由于脂质的存在导致粉末结块。文中讨论了缓解这些问题的方法。
