Wehling R L, Wetzel D L, Pedersen J R
J Assoc Off Anal Chem. 1984 May-Jun;67(3):644-7.
Liquid chromatography is used to measure the uric acid content of wheat internally infested by various growth stages of granary weevil (Sitophilus granarius), rice weevil (Sitophilus oryzae), and lesser grain borer (Ryzopertha dominica). Good correlation exists between numbers of insects and uric acid content of grain infested by a given stage of an internally infesting stored product insect, with correlation coefficients ranging from 0.970 to 0.998. Uric acid content of infested grain increases as the insect life cycle progresses, with granary weevil producing the greatest quantities of uric acid followed in order by rice weevil and lesser grain borer. A detection limit for the analytical procedure of less than 1.0 ppm uric acid allows detection of infestation levels as low as one kernel per 100 g grain for late instar granary weevil larvae. This limit is sufficiently sensitive to detect levels of infestation that may be commonly encountered in commerce.
液相色谱法用于测定受谷象（Sitophilus granarius）、米象（Sitophilus oryzae）和谷蠹（Ryzopertha dominica）不同生长阶段侵染的小麦中的尿酸含量。对于受特定阶段内寄生性储粮害虫侵染的谷物，昆虫数量与谷物中尿酸含量之间存在良好的相关性，相关系数范围为0.970至0.998。随着昆虫生命周期的推进，受侵染谷物的尿酸含量会增加，谷象产生的尿酸量最大，其次是米象和谷蠹。该分析方法的检测限低于1.0 ppm尿酸，这使得能够检测到低至每100克谷物中有一粒谷象老龄幼虫的侵染水平。这个限度足够灵敏，能够检测到商业中可能常见的侵染水平。
