Eisenstein R S, Harper A E
J Biol Chem. 1984 Aug 10;259(15):9922-8.
An in vitro system prepared from rat liver postmitochondrial supernatant exhibits a high rate of protein synthesis for an extended period of time. This system initiates translation of either endogenous or exogenous mRNA, incorporates Met at a rate of 13 pmol/mg of postmitochondrial supernatant protein/min, maintains this rate for at least 90 min, and performs several rounds of translation/mRNA molecule. Up to 50% of the activity is due to reinitiation of protein synthesis using endogenous mRNA. In addition, 60-70% of the protein synthesized was released from ribosomes into the medium. Addition of globin mRNA stimulates protein synthesis and results in the synthesis of a protein that comigrates with authentic rabbit globin. Black beetle virus mRNA 2 also stimulates protein synthesis and results in synthesis of a protein with molecular weight corresponding to that of the mature viral protein. With endogenous rat liver mRNA this system synthesizes a large number of proteins.
由大鼠肝脏线粒体后上清液制备的体外系统在较长时间内呈现出较高的蛋白质合成速率。该系统可起始内源性或外源性mRNA的翻译，以13 pmol/毫克线粒体后上清液蛋白/分钟的速率掺入甲硫氨酸，将此速率维持至少90分钟，并对每个mRNA分子进行多轮翻译。高达50%的活性归因于利用内源性mRNA重新起始蛋白质合成。此外，所合成蛋白质的60 - 70%从核糖体释放到培养基中。添加珠蛋白mRNA可刺激蛋白质合成，并导致合成一种与天然兔珠蛋白迁移率相同的蛋白质。黑甲虫病毒mRNA 2也刺激蛋白质合成，并导致合成一种分子量与成熟病毒蛋白相对应的蛋白质。利用大鼠肝脏内源性mRNA，该系统可合成大量蛋白质。
