Raap A K, Van Duijn P
J Histochem Cytochem. 1984 Aug;32(8):815-20. doi: 10.1177/32.8.6747273.
Erythrocyte ghosts containing varying amounts of alkaline phosphatase were used to study the localization mechanisms of three metal salt and one azo method for this enzyme. For the azo method, the minimal amount of alkaline phosphatase that can be visualized within the ghosts proved only to be limited by the optical properties of the azo compound. In contrast, for the metal salt methods, a certain threshold activity had to be present in the ghosts in order to obtain correct localization of the final reaction product. The localization properties of both azo and metal salt methods conformed to the theories of cytochemical enzyme localization presented to date. By determining the rate constant of the capture reaction and the diffusion constant of the primary product, the localization properties of the azo method could be predicted. Some remaining discrepancies between theory and practice are discussed.
使用含有不同量碱性磷酸酶的红细胞血影来研究三种金属盐法和一种偶氮法对该酶的定位机制。对于偶氮法，在血影内能够可视化的碱性磷酸酶的最小量仅受偶氮化合物光学性质的限制。相比之下，对于金属盐法，血影中必须存在一定的阈值活性才能获得最终反应产物的正确定位。偶氮法和金属盐法的定位特性均符合迄今为止提出的细胞化学酶定位理论。通过确定捕获反应的速率常数和初级产物的扩散常数，可以预测偶氮法的定位特性。讨论了理论与实践之间一些仍然存在的差异。
