Prosperi E, Raap A K, van der Ploeg M
Histochemistry. 1982;75(3):387-97. doi: 10.1007/BF00496741.
The reliability of histochemical determinations of the enzyme activity after thermal damage has been studied with the aid of two model systems. Polyacrylamide films and erythrocyte ghosts containing either beta-glucuronidase or alkaline phosphatase, were submitted to heating and the activities retained were assessed both biochemically and histochemically. For the enzymes studied, the results show that tissue alterations induced by heat can influence histochemical reaction procedures, and that with these model systems, factors which are important for the histochemical quantitation of enzyme activities in thermally damaged tissues can be evaluated quantitatively. Potentialities of these model systems in the study of evaluating thermal damage through histochemical enzyme activity determinations, are discussed.
借助两个模型系统研究了热损伤后酶活性组织化学测定的可靠性。将含有β-葡萄糖醛酸酶或碱性磷酸酶的聚丙烯酰胺膜和红细胞血影进行加热，然后通过生化和组织化学方法评估保留的活性。对于所研究的酶，结果表明热诱导的组织改变会影响组织化学反应过程，并且利用这些模型系统，可以对热损伤组织中酶活性组织化学定量的重要因素进行定量评估。讨论了这些模型系统在通过组织化学酶活性测定评估热损伤研究中的潜力。
