大鼠和小鼠脑组织中1-甲基咪唑-4-乙酸的测定

Determination of 1-methylimidazole-4-acetic acid in brain tissue from rat and mouse.

作者信息

Swahn C G

出版信息

J Neurochem. 1984 Sep;43(3):830-4. doi: 10.1111/j.1471-4159.1984.tb12805.x.

DOI:10.1111/j.1471-4159.1984.tb12805.x

Abstract

The concentration of the histamine metabolite 1-methylimidazole-4-acetic acid was determined in brain tissue from rat and mouse with a gas chromatographic-mass spectrometric method. Mouse brain contained 1.7-3.2 nmol/g, depending on the strain. The concentration in cerebrum from Sprague-Dawley rats was 1.2 nmol/g, whereas cerebellum contained 0.24 nmol/g. The concentration of tele-methylhistamine in mouse brain was 1.4-2.2 nmol/g. The concentration of 1-methylimidazole-4-acetic acid in rat brain after death did not change significantly during 2 h at room temperature.

摘要

采用气相色谱-质谱联用法测定了大鼠和小鼠脑组织中组胺代谢产物1-甲基咪唑-4-乙酸的浓度。小鼠脑内该物质的含量为1.7 - 3.2纳摩尔/克，具体数值取决于品系。斯普拉格-道利大鼠大脑中的浓度为1.2纳摩尔/克，而小脑的浓度为0.24纳摩尔/克。小鼠脑中甲基组胺的浓度为1.4 - 2.2纳摩尔/克。大鼠死后，脑内1-甲基咪唑-4-乙酸的浓度在室温下2小时内无显著变化。