Bachelard H S, Cox D W, Drower J
J Physiol. 1984 Jul;352:91-102. doi: 10.1113/jphysiol.1984.sp015279.
Evoked granule cell field potentials, and levels of tissue metabolites, in superfused guinea-pig hippocampal slices have been studied in the presence of low glucose and an alternative glycolytic substrate (D-fructose). The effects of glucose analogues (5-thio-D-glucose, 2-deoxy-D-glucose or 3-O-methyl-D-glucose) in the presence of glucose were also tested. Concentrations of glucose or fructose in excess of 2 mM and 10 mM respectively were required to maintain normal evoked activity. 5-Thioglucose (15 mM) in the presence of 5 mM-glucose decreased the amplitude of the population spike by 60% with little effect on population excitatory post-synaptic potential (e.p.s.p.). Tissue levels of phosphocreatine and ATP were essentially unchanged under all conditions tested, with the exception of 10 mM-fructose. The decrease in rates of lactate efflux from superfused tissue during and after superfusion with 3-O-methylglucose, 2-deoxyglucose or 5-thioglucose was found to be positively correlated with the extent of attenuation of field potentials. Analysis of the relationship between population spike amplitude and rates of rise of e.p.s.p., under conditions where field potentials were attenuated, showed that the population spike was always more sensitive to metabolic perturbation than was the e.p.s.p., thus indicating an effect on cell excitability. It is suggested that some aspect of non-oxidative glucose metabolism is important in maintaining this granule cell excitability.
在低葡萄糖和替代糖酵解底物（D-果糖）存在的情况下，研究了豚鼠海马脑片灌流液中的诱发性颗粒细胞场电位和组织代谢物水平。还测试了葡萄糖类似物（5-硫代-D-葡萄糖、2-脱氧-D-葡萄糖或3-O-甲基-D-葡萄糖）在葡萄糖存在时的作用。分别需要超过2 mM的葡萄糖和10 mM的果糖才能维持正常的诱发性活动。5 mM葡萄糖存在时加入15 mM 5-硫代葡萄糖，可使群体峰电位幅度降低60%，而对群体兴奋性突触后电位（e.p.s.p.）影响很小。除10 mM果糖外，在所有测试条件下，磷酸肌酸和ATP的组织水平基本保持不变。发现在用3-O-甲基葡萄糖、2-脱氧葡萄糖或5-硫代葡萄糖灌流期间和之后，灌流组织中乳酸流出速率的降低与场电位衰减程度呈正相关。在场电位衰减的条件下，分析群体峰电位幅度与e.p.s.p.上升速率之间的关系，结果表明群体峰电位对代谢扰动总是比e.p.s.p.更敏感，从而表明对细胞兴奋性有影响。有人认为非氧化葡萄糖代谢的某些方面对维持这种颗粒细胞兴奋性很重要。
