Kanatani T, Mizuno K, Okada Y
Department of Physiology, Kobe University, School of Medicine, Japan.
Experientia. 1995 Mar 15;51(3):213-6. doi: 10.1007/BF01931098.
The present study was undertaken to investigate whether neural activity of hippocampal slices can be preserved after replacing D-glucose with glycolytic intermediate metabolites such as lactate, pyruvate and citrate or with other sugars such as fructose, mannose, maltose, glucosamine, sucrose and galactose. As an index of neural activity, population spikes (PS) were recorded in the granule cell layers after electrical stimulation to the perforant path of guinea pig hippocampal slices. In addition, we determined the levels of ATP and creatine phosphate (CrP) in each slice after the replacement of D-glucose with these substrates, and correlated it with the neural activity. Substrates other than D-glucose could not maintain the PS for even 20 min although the slices perfused with medium containing lactate, pyruvate, galactose, fructose and maltose maintained similar levels of ATP and CrP as in slices incubated in the D-glucose-containing medium. These results indicate that D-glucose is essential for the preservation of synaptic activity in addition to its main role as the substrate for energy production to maintain the levels of high energy phosphates.
本研究旨在探讨用乳酸、丙酮酸和柠檬酸等糖酵解中间代谢产物或果糖、甘露糖、麦芽糖、氨基葡萄糖、蔗糖和半乳糖等其他糖类替代D-葡萄糖后，海马切片的神经活动是否能够得以保留。作为神经活动的指标，在对豚鼠海马切片的穿通路径进行电刺激后，在颗粒细胞层记录群体锋电位（PS）。此外，在用这些底物替代D-葡萄糖后，我们测定了每片切片中ATP和磷酸肌酸（CrP）的水平，并将其与神经活动相关联。尽管灌注含有乳酸、丙酮酸、半乳糖、果糖和麦芽糖培养基的切片维持了与在含D-葡萄糖培养基中孵育的切片相似的ATP和CrP水平，但除D-葡萄糖外的其他底物甚至无法维持PS达20分钟。这些结果表明，D-葡萄糖除了作为产生能量以维持高能磷酸盐水平的底物这一主要作用外，对于保留突触活动也是必不可少的。
