Valentijn R M, van Es L A, Daha M R
J Clin Lab Immunol. 1984 Jun;14(2):81-6.
Four immune complex assays (PEG assays) are described; they are based on the binding of radio-labelled immunospecific antibodies to immune complexes containing IgG, IgA, C3 or C4, and subsequent precipitation with polyethylene glycol. Comparison of the results of the IgA-PEG assay with those of an existing immune complex assay (alpha-IgA-InhBA) showed that the former detects only large-sized (greater than 25 S) material. This was also demonstrated by sucrose density gradient ultracentrifugation of immune complexes in patients' sera as well as in preparations of aggregates containing IgA, C3 or C4. By using aggregates of mixed composition (IgG, IgA, C3 and C4) it was shown that each constituent could be detected by one of the 4 assays. Immune complexes containing the various constituents could also be detected in sera of patients with a variety of disorders. Further studies are needed to establish whether these constituents occur within the same complex or within different complexes.
本文描述了四种免疫复合物检测方法（聚乙二醇检测法）；这些方法基于放射性标记的免疫特异性抗体与含有IgG、IgA、C3或C4的免疫复合物的结合，随后用聚乙二醇进行沉淀。将IgA-聚乙二醇检测法的结果与现有的免疫复合物检测法（α-IgA-InhBA）的结果进行比较，发现前者仅检测大尺寸（大于25S）的物质。这也通过对患者血清中的免疫复合物以及含有IgA、C3或C4的聚集体制剂进行蔗糖密度梯度超速离心得到了证实。通过使用混合成分（IgG、IgA、C3和C4）的聚集体，结果表明每种成分都可以通过这四种检测方法中的一种进行检测。在患有各种疾病的患者血清中也可以检测到含有各种成分的免疫复合物。需要进一步研究来确定这些成分是存在于同一复合物中还是不同的复合物中。
