Richardson W D, Anderson C W
J Virol. 1984 Aug;51(2):559-62. doi: 10.1128/JVI.51.2.559-562.1984.
Adenovirus 2-infected monkey cells fail to synthesize fiber, a 62,000 Mr virion polypeptide expressed at late times in productively infected cells. Yet these cells contain fiber mRNA that, after isolation, can be translated in vitro. The reason for the failure of monkey cells to translate fiber mRNA has been approached by microinjecting adenovirus mRNA into the cytoplasm of cultured monkey cells. Late adenovirus 2 mRNA, isolated from infected HeLa cells, was efficiently expressed when microinjected into the African green monkey kidney cell line CV-C. Expressed viral proteins identified by immunoprecipitation included the adenovirus fiber polypeptide. This result demonstrates that the monkey cell translational apparatus is capable of recognizing and expressing functional adenovirus fiber mRNA. Microinjection of late virus mRNA into cells previously infected with wild-type adenovirus 2 failed to increase significantly the yield of infectious virus.
腺病毒2感染的猴细胞无法合成纤维蛋白，这是一种在高效感染细胞中晚期表达的62000道尔顿的病毒粒子多肽。然而，这些细胞含有纤维蛋白mRNA，分离后可在体外进行翻译。通过将腺病毒mRNA显微注射到培养的猴细胞细胞质中，探讨了猴细胞无法翻译纤维蛋白mRNA的原因。从感染的HeLa细胞中分离出的晚期腺病毒2 mRNA，显微注射到非洲绿猴肾细胞系CV-C中时能高效表达。通过免疫沉淀鉴定出的表达病毒蛋白包括腺病毒纤维多肽。这一结果表明，猴细胞翻译装置能够识别并表达功能性腺病毒纤维蛋白mRNA。将晚期病毒mRNA显微注射到先前感染野生型腺病毒2的细胞中，未能显著提高感染性病毒的产量。
