Anderson K P, Klessig D F
J Virol. 1982 May;42(2):748-54. doi: 10.1128/JVI.42.2.748-754.1982.
Northern (RNA) blot analysis has been used to show that synthesis of early mRNA species is similar in monkey cells productively or abortively infected with human adenovirus. mRNA species from all five major early regions (1A, 1B, 2, 3, 4) are identical in size and comparable in abundance whether isolated from monkey cells infected with adenovirus type 2 or with the host range mutant Ad2hr400 or coinfected with adenovirus type 2 plus simian virus 40. The mRNA species isolated from monkey cells are identical in size to those isolated from human cells. Production of virus-associated RNA is also identical in productive and abortive infections of monkey cells. Synthesis of virus-associated RNA is, however, significantly greater in HeLa cells than in CV1 cells at late times after infection regardless of which virus is used in the infection.
Northern(RNA)印迹分析已被用于表明,在被人腺病毒有效感染或流产感染的猴细胞中,早期mRNA种类的合成是相似的。无论从感染2型腺病毒、宿主范围突变体Ad2hr400的猴细胞中分离,还是从同时感染2型腺病毒和猿猴病毒40的猴细胞中分离,来自所有五个主要早期区域(1A、1B、2、3、4)的mRNA种类在大小上相同,丰度也相当。从猴细胞中分离的mRNA种类在大小上与从人细胞中分离的mRNA种类相同。在猴细胞的有效感染和流产感染中,病毒相关RNA的产生也是相同的。然而,在感染后的晚期,无论感染使用哪种病毒,HeLa细胞中病毒相关RNA的合成都比CV1细胞中的显著更多。