Nakano T, Kawai S, Nagatsu T
Jpn J Pharmacol. 1984 Jun;35(2):163-7. doi: 10.1254/jjp.35.163.
A new fluorometric assay for the determination of monoamine oxidase activity that is applicable to any substrates including dopamine and serotonin is described. Hydrogen peroxide formed during the monoamine oxidase reaction was reduced in the presence of glutathione and glutathione peroxidase, and the oxidized glutathione was measured fluorometrically as NADP+ via oxidation of NADPH by glutathione reductase. This method was applied for inhibitor studies using clorgyline and deprenyl.
描述了一种用于测定单胺氧化酶活性的新型荧光测定法，该方法适用于包括多巴胺和血清素在内的任何底物。在谷胱甘肽和谷胱甘肽过氧化物酶存在的情况下，单胺氧化酶反应过程中形成的过氧化氢被还原，氧化型谷胱甘肽通过谷胱甘肽还原酶将NADPH氧化而以NADP⁺的形式进行荧光测定。该方法应用于使用氯吉兰和司来吉兰的抑制剂研究。
