Few J D, Chaudry S, James V H
J Steroid Biochem. 1984 Jul;21(1):87-92. doi: 10.1016/0022-4731(84)90064-5.
A method is described for the direct radioimmunoassay of aldosterone in human saliva without preliminary extraction or chromatographic purification. The method has been validated with respect to sensitivity, accuracy, precision, specificity and other criteria. Under basal conditions concentrations of aldosterone in saliva ranged from 10-63% those of plasma (mean +/- SD = 32.5 +/- 13.1%). When plasma aldosterone responded to such acute stimuli as postural change, exercise or ACTH infusion, this was promptly reflected in corresponding changes in saliva aldosterone concentration. In subjects going about their normal daily business saliva aldosterone concentration fluctuated markedly during the day. It is therefore difficult to define a normal range for saliva aldosterone concentration but present observations suggest an upper limit of 150 pmol/l under basal conditions.
本文描述了一种无需预先提取或色谱纯化即可直接放射免疫测定人唾液中醛固酮的方法。该方法已在灵敏度、准确性、精密度、特异性及其他标准方面得到验证。在基础条件下，唾液中醛固酮浓度为血浆浓度的10 - 63%（平均值±标准差 = 32.5 ± 13.1%）。当血浆醛固酮对诸如体位改变、运动或促肾上腺皮质激素输注等急性刺激产生反应时，唾液醛固酮浓度会相应迅速变化。在正常进行日常活动的受试者中，唾液醛固酮浓度在一天中波动明显。因此，很难确定唾液醛固酮浓度的正常范围，但目前的观察结果表明基础条件下上限为150 pmol/l。
