Müller-Peddinghaus R, Erdtmann K
Methods Find Exp Clin Pharmacol. 1984 Jun;6(6):287-92.
The combination of the two well known in vitro methods of hypotonic shock and immune hemolysis employing sheep erythrocytes (E) were used to evaluate membrane stabilization. Direct drug-induced hemolysis of antibody-coated E (EA), protection against hypotonic shock and complement (C)-mediated hemolysis can be recorded from a consecutive preparative procedure to give information to irreversible drug effects. The most active probable working mechanisms are interference with binding sites of the complement membrane attack complex (MAC) and unspecific membrane stabilization. Protection against C-mediated erythrolysis appeared more sensitive than against hypotonic shock.
采用两种众所周知的体外方法，即低渗休克法和使用绵羊红细胞（E）的免疫溶血法相结合，来评估膜稳定性。从连续的制备过程中可以记录到药物直接诱导的抗体包被红细胞（EA）溶血、对低渗休克的保护作用以及补体（C）介导的溶血，以获取有关药物不可逆作用的信息。最可能的作用机制是干扰补体膜攻击复合物（MAC）的结合位点和非特异性膜稳定作用。对C介导的红细胞溶解的保护作用似乎比对低渗休克的保护作用更敏感。
