Verburg K M, Bowsher R R, Henry D P
Life Sci. 1984 Jul 16;35(3):241-51. doi: 10.1016/0024-3205(84)90107-3.
The specificity of the histamine N-methyltransferase (HNMT) based radioenzymatic assay for histamine has been questioned since N-alpha-methylhistamine is also a substrate for this enzyme. Purification of HNMT for use in the radioenzymatic assay improves sensitivity and specificity of this procedure. In this investigation, further improvements in specificity, with respect to other HNMT substrates, were attained by optimization of reaction conditions based on the evaluation of HNMT kinetic parameters. These studies demonstrate that appropriate control of reaction temperature and concentration of both the enzyme and the radiolabeled methyl donor improve the specificity of this assay for histamine.
由于N-α-甲基组胺也是组胺N-甲基转移酶（HNMT）的底物，基于该酶的组胺放射酶法的特异性受到了质疑。用于放射酶法的HNMT纯化提高了该方法的灵敏度和特异性。在本研究中，通过基于HNMT动力学参数评估优化反应条件，在针对其他HNMT底物的特异性方面取得了进一步的改进。这些研究表明，适当控制反应温度以及酶和放射性标记甲基供体的浓度可提高该组胺测定法的特异性。
