Hörauf A M, Matek M, Raithel M, Baenkler H W
Institute of Clinical Immunology and Rheumatology, University of Erlangen-Nürnberg, FRG.
Agents Actions. 1989 Apr;27(1-2):89-92. doi: 10.1007/BF02222207.
Testing of tissue particles for mediator release may be very useful for the diagnosis of localized immunological abnormalities or allergies. The aim of this study was to set up a general procedure to test the reaction of large bowel mucosa to stimuli via the IgE-mediated pathway. Therefore, tissue particles from normal subjects and from patients suffering from different diseases (Crohn's disease, ulcerative colitis, intestinal polyps) obtained at routine coloscopy were exposed to either Hanks or anti-IgE solution to determine the spontaneous or the anti-IgE-induced histamine release, expressed as the percentage of the total histamine content of the biopsy. Histamine was measured using the single isotope radioenzymatic assay. In general, whereas anti-IgE interestingly reduced the histamine release compared to the spontaneous in most of the patients within the polyps group, there was a stimulating effect of anti-IgE throughout all other groups. Thus, the study confirms the possibility of performing functional tests using biopsy particles from the colon.
检测组织颗粒的介质释放对于诊断局部免疫异常或过敏可能非常有用。本研究的目的是建立一种通用程序，以通过IgE介导的途径测试大肠黏膜对刺激的反应。因此，在常规结肠镜检查时从正常受试者和患有不同疾病（克罗恩病、溃疡性结肠炎、肠息肉）的患者获取的组织颗粒，分别暴露于汉克斯溶液或抗IgE溶液中，以确定自发的或抗IgE诱导的组胺释放，以活检组织中总组胺含量的百分比表示。组胺采用单同位素放射酶法测定。总体而言，息肉组中的大多数患者，与自发组胺释放相比，抗IgE有趣地降低了组胺释放，而在所有其他组中抗IgE均有刺激作用。因此，该研究证实了使用结肠活检颗粒进行功能测试的可能性。
