Bochkov N P, Chebotarev A N, Filippova T V, Platonova V I, Stukalov S V, Debova G A
Mutat Res. 1984 Jul;127(2):149-53. doi: 10.1016/0027-5107(84)90016-2.
A significant decrease in the baseline of sister-chromatid exchanges (SCEs) was observed in cultured human lymphocytes, if 5-bromodeoxyuridine (BrdU) was added after 60 h of culture, and the cells were harvested at least 24-30 h after BrdU exposure. This decrease is supposed to occur if at least one cell division takes place before the addition of BrdU. For cytogenetic monitoring of mutagenic environmental factors, using human lymphocyte cultures, it is assumed that two time periods are sufficient for comparison.
在培养的人淋巴细胞中观察到，如果在培养60小时后添加5-溴脱氧尿苷（BrdU），并且在BrdU暴露后至少24 - 30小时收获细胞，则姐妹染色单体交换（SCE）的基线会显著降低。如果在添加BrdU之前至少发生一次细胞分裂，这种降低就应该会发生。对于使用人淋巴细胞培养物对诱变环境因素进行细胞遗传学监测，假定两个时间段就足以进行比较。
