Giulotto E, Mottura A, Giorgi R, de Carli L, Nuzzo F
Mutat Res. 1980 May;70(3):343-50. doi: 10.1016/0027-5107(80)90024-x.
The frequency of sister-chromatid exchanges (SCE) was determined on second-division metaphases of lymphocytes stimulated by phytohaemagglutinin (PHA) during 9 days of culture. By using either a continuous or a pulsed bromodeoxyuridine (BUdR) treatment, cells were selected that had divided only twice, or at least twice, after different culture periods. No significant differences were observed in the SCE frequencies among the various samples. The incidence of SCE appears to be independent of the proliferation properties of cultured lymphocytes, such as length of cell cycle, fast or delayed response to PHA and number of divisions performed in vitro.
在培养9天期间，通过对经植物血凝素（PHA）刺激的淋巴细胞的第二次分裂中期进行分析，测定姐妹染色单体交换（SCE）的频率。通过连续或脉冲式溴脱氧尿苷（BUdR）处理，选择在不同培养期后仅分裂两次或至少分裂两次的细胞。在各个样本之间，未观察到SCE频率有显著差异。SCE的发生率似乎与培养淋巴细胞的增殖特性无关，如细胞周期长度、对PHA的快速或延迟反应以及体外分裂次数。
