Suslov I, McCoy J L, Herberman R B
J Natl Cancer Inst. 1981 Feb;66(2):233-7.
Mononuclear (MN) cells from the peripheral blood of lung cancer patients were tested for their ability to respond to a 3-M KCl extract of adenocarcinoma of lung with the use of an indirect leukocyte migration inhibition (LMl) assay. Antigen-stimulated MN cell cultures were evaluated for leukocyte inhibitory factor production by their ability to inhibit the migration of indicator polymorphonuclear cells from agarose droplets. When supernatants were prepared in conventional round-bottomed tubes (5X10(6) cell/tube), 25 of 44 (57%) lung cancer patients had positive indirect LMl responses to the 7661 antigen as compared to only 2 of the 30 (7%) normal donors. When supernatants were prepared in conical microtubes, with 10 times fewer MN cells, similar results were obtained. Patients with all histologic types of lung cancer had a similar incidence of reactivity, and reactivity of untreated patients did not appear to be related to stage of disease or degree of tumor burden. Surgical removal of the tumor appeared to decrease the incidence of reactivity in the 1- to 12-month postoperative period. These results strongly suggest that the LMl reactivity against lung tumor extracts is lymphokine mediated, inducing cellular responses by the patients against such antigens.
利用间接白细胞迁移抑制（LMI）试验，检测肺癌患者外周血中的单核细胞（MN）对肺腺癌3-M KCl提取物的反应能力。通过抗原刺激的MN细胞培养物抑制指示性多形核细胞从琼脂糖滴中迁移的能力，来评估白细胞抑制因子的产生。当在传统的圆底管（每管5×10⁶个细胞）中制备上清液时，44例肺癌患者中有25例（57%）对7661抗原有阳性间接LMI反应，而30例正常供者中只有2例（7%）有阳性反应。当在锥形微量管中制备上清液时，MN细胞数量减少10倍，也获得了类似的结果。所有组织学类型的肺癌患者的反应发生率相似，未治疗患者的反应性似乎与疾病分期或肿瘤负荷程度无关。手术切除肿瘤似乎会降低术后1至12个月内的反应发生率。这些结果强烈表明，针对肺肿瘤提取物的LMI反应性是由淋巴因子介导的，可诱导患者针对此类抗原产生细胞反应。
