Fromtling R A, Shadomy H J, Jacobson E S
Mycopathologia. 1982 Jul 23;79(1):23-9. doi: 10.1007/BF00636177.
Six acapsular strains of Cryptococcus neoformans obtained by chemical mutagenesis failed to produce a capsule in vivo and were avirulent in mice following high dose intramuscular, intraperitoneal or intravenous inoculation. Peritoneal granulomas were observed in all animals inoculated with the acapsular mutants. These granulomas were characterized by a large central mass consisting of intact, degenerating and necrotic yeast cells. This was surrounded by concentric layers of a broad band of histiocytes, a narrow band of fibroblasts, and around the periphery, a mass of lymphocytes and plasma cells. These isolates did not revert to an encapsulated or virulent state after more than a year of subculturing or 18 passages through mice.
通过化学诱变获得的6株新型隐球菌无荚膜菌株在体内无法产生荚膜，在高剂量肌肉注射、腹腔注射或静脉注射后对小鼠无致病性。在所有接种无荚膜突变体的动物中均观察到腹膜肉芽肿。这些肉芽肿的特征是中央有一大团由完整、退化和坏死的酵母细胞组成的物质。其周围是由一层宽的组织细胞带、一层窄的成纤维细胞带同心排列而成，在外围是大量淋巴细胞和浆细胞。经过一年多的传代培养或在小鼠体内传代18次后，这些分离株未恢复为有荚膜或有致病性的状态。
