Hrmová M, Drobnica L
Mycopathologia. 1982 Jul 23;79(1):55-64. doi: 10.1007/BF00636183.
Two new effective methods for synchronous germ tube production in Candida albicans have been described. Both are based on the use of stationary grown cultures and their further incubation in an aerated simple mineral medium enriched with vitamins containing either high glucose concentration (100 mmol/1) and the antibiotic monorden being added, or N-acetyl-D-glucosamine (100 mmol/1) as the sole carbon source. On the other hand yeast morphology could be maintained in the medium with high glucose concentration. On the basis of the methods developed it was possible to compare respiration intensity, respiration quotients, and sensitivity against some metabolic inhibitors in both morphological forms. Labeling experiments showed slight differences in the time course of glycine incorporation. The mycelial cell walls contained more chitin than the yeastlike cells. Using light and electron microscopy the interrelationships between concentration of monorden, or N-acetyl-D-glucosamine, physiological state of inoculum and the germ tube frequency were determined. The results are discussed with regard to the induction of germ tubes by low glucose concentration in Candida albicans from the more general aspect of regulation of fungal morphogenesis.
已描述了两种在白色念珠菌中同步产生芽管的新有效方法。这两种方法均基于使用静置培养的培养物，并将其在富含维生素的通气简单矿物培养基中进一步培养，该培养基含有高葡萄糖浓度（100 mmol/1）并添加抗生素莫能菌素，或使用N-乙酰-D-葡萄糖胺（100 mmol/1）作为唯一碳源。另一方面，在高葡萄糖浓度的培养基中可以维持酵母形态。基于所开发的方法，可以比较两种形态形式下的呼吸强度、呼吸商以及对某些代谢抑制剂的敏感性。标记实验表明甘氨酸掺入的时间进程存在细微差异。菌丝细胞壁比酵母样细胞含有更多的几丁质。使用光学显微镜和电子显微镜确定了莫能菌素或N-乙酰-D-葡萄糖胺的浓度、接种物的生理状态与芽管频率之间的相互关系。从真菌形态发生调控的更一般方面，讨论了白色念珠菌中低葡萄糖浓度诱导芽管形成的结果。
