Chaffin W L, Sogin S J
J Bacteriol. 1976 May;126(2):771-6. doi: 10.1128/jb.126.2.771-776.1976.
Homogenous cell populations of increasing cell volume may have been isolated from exponential and stationary culture of Candida albicans by centrifugation on a sucrose gradient. Observations of the yeast-mycelial transition using these populations showed the following. (i) No fraction from early logarithmic phase cells was unable to undergo morphological transition. (ii) The time of initiation of germ tube production was correlated with cell size in stationary-phase cultures. (iii) The rate of appearance of germ tubes was nearly identical in all fractions measured. (iv) Addition of N-acetyl-D-glucosamine to homogeneous cell populations decreased the time of initial appearance of germ tubes but did not affect the rate of appearance after initiation.
通过在蔗糖梯度上离心,可能已从白色念珠菌的指数生长期和稳定期培养物中分离出细胞体积不断增加的同质细胞群体。使用这些群体对酵母-菌丝体转变进行的观察结果如下:(i)对数生长期早期细胞的任何组分都能够进行形态转变。(ii)在稳定期培养物中,芽管产生起始时间与细胞大小相关。(iii)在所有测定的组分中,芽管出现的速率几乎相同。(iv)向同质细胞群体中添加N-乙酰-D-葡萄糖胺可缩短芽管最初出现的时间,但不影响起始后芽管出现的速率。