Ritchie T C, Westlund K N, Bowker R M, Coulter J D, Leonard R B
Neuroscience. 1982 Jun;7(6):1471-82. doi: 10.1016/0306-4522(82)90258-5.
We have re-examined in the rat the nuclear localization of the medullary catecholamine-containing cell groups (A1 and A2) and their relation to the vagal motor nuclei using a double labeling method. The vagal nuclei were defined by the retrograde transport of horseradish peroxidase applied to the cervical vagus, and noradrenergic and adrenergic neurons were stained with the peroxidase-antiperoxidase immunocytochemical method using an antibody to dopamine beta-hydrolase. The method allows visualization of both labels within single neurons. The neurons of the A2 group are primarily distributed in both the nucleus of the solitary tract and the dorsal motor nucleus of the vagus in a complex interrelationship that depends on the rostrocaudal level. Caudal to the obex, cells of the dorsal motor nucleus of the vagus are scattered among cells immunoreactive for dopamine beta-hydroxylase in the area considered to be the commissural subnucleus of the nucleus of the solitary tract. At levels near and slightly rostral to the obex, the dopamine beta-hydroxylase-positive cells are largely confined to nucleus of the solitary tract. However, the rostral third of the A2 group lies predominantly within dorsal motor nucleus, as defined by horseradish peroxidase labeled cells, with only a few cells in the nucleus of the solitary tract. A subset of the dopamine beta-hydroxylase positive cells within the rostral dorsal motor nucleus of the vagus are also vagal efferents. Our results suggest that a second population of dopamine beta-hydroxylase positive vagal efferents may exist ventrolaterally where neurons of the AI cell group intermingle with those of nucleus ambiguus.
我们使用双重标记法在大鼠中重新检查了含儿茶酚胺的延髓细胞群（A1和A2）的核定位及其与迷走运动核的关系。通过将辣根过氧化物酶逆行运输至颈迷走神经来确定迷走神经核，并用抗多巴胺β-羟化酶抗体通过过氧化物酶-抗过氧化物酶免疫细胞化学方法对去甲肾上腺素能和肾上腺素能神经元进行染色。该方法可使单个神经元内的两种标记物都可视化。A2组的神经元主要分布在孤束核和迷走神经背运动核中，其复杂的相互关系取决于前后水平。在闩尾侧，迷走神经背运动核的细胞散布在被认为是孤束核连合亚核区域内对多巴胺β-羟化酶免疫反应阳性的细胞之间。在接近闩和略高于闩的水平，多巴胺β-羟化酶阳性细胞主要局限于孤束核。然而，A2组的头侧三分之一主要位于由辣根过氧化物酶标记的细胞所定义的迷走神经背运动核内，孤束核中只有少数细胞。迷走神经头侧背运动核内多巴胺β-羟化酶阳性细胞的一个亚群也是迷走神经传出纤维。我们的结果表明，在腹外侧可能存在第二群多巴胺β-羟化酶阳性的迷走神经传出纤维，此处A1细胞群的神经元与疑核的神经元相互混合。
