Jirikowski G, Reisert I, Pilgrim C
Neurosci Lett. 1982 Jul 20;31(1):75-9. doi: 10.1016/0304-3940(82)90057-x.
Immunocytochemistry using the unlabeled antibody (PAP) technique reveals the presence of 0.01-0.3% neurons reacting with an antiserum against vasoactive intestinal polypeptide (VIP) in dissociation cultures of hypothalamus and midbrain. The cultures are obtained from 18-day-old rat fetuses and maintained for 22 and 29 days in vitro. Most of the neurons appear well differentiated giving off branched processes with pronounced varicosities. These can often be seen to contact perikarya as well as processes of unstained neurons.
使用未标记抗体（PAP）技术的免疫细胞化学显示，在来自18日龄大鼠胎儿的下丘脑和中脑解离培养物中，有0.01 - 0.3%的神经元与抗血管活性肠多肽（VIP）抗血清发生反应。这些培养物在体外维持22天和29天。大多数神经元看起来分化良好，发出带有明显膨体的分支突起。这些突起常常可见与未染色神经元的胞体以及突起相接触。
