Borzini P, Aniasi A, DeGiacomo M, Bersani L, Sirchia G
J Clin Immunol. 1982 Jul;2(3 Suppl):95S-102S.
Manual procedures to evaluate mononuclear cells reacted with monoclonal antibodies were evaluated with respect to reproducibility, ease of execution, and cost. The immunofluorescent technique and the complement-mediated cytotoxicity test produced very similar results; however, the latter was clearly preferable in terms of cost, time-consumption, and ease of execution. Evidence is also presented that purification of lymphocytes using gradient diffusion techniques may induce an uneven selectivity within the relative percentages of T cell subsets collected for analysis.
针对评估与单克隆抗体反应的单核细胞的手工操作程序，在可重复性、操作简便性和成本方面进行了评估。免疫荧光技术和补体介导的细胞毒性试验产生了非常相似的结果；然而，就成本、耗时和操作简便性而言，后者显然更具优势。还有证据表明，使用梯度扩散技术纯化淋巴细胞可能会在收集用于分析的T细胞亚群的相对百分比内诱导不均匀的选择性。
