利用单克隆抗体双染色技术描绘人类胸腺细胞分化途径。

Tidman N, Janossy G, Bodger M, Granger S, Kung P C, Goldstein G

Clin Exp Immunol. 1981 Sep;45(3):457-67.

Three new techniques have been introduced for the phenotypic analysis of human thymocytes and T cells. Monoclonal antibodies of different subclasses (i.e. IgG1 and IgG2) were used in various combinations and labelled with subclass-specific second layers conjugated to different fluorochromes. Tissue sections were studied with combinations of monoclonal and conventional reagents, and anti-mouse Ig-coated macrobead columns were used to separate antibody-tagged cells. With the help of these simple techniques, four stage of thymocyte differentiation (prothymocyte, cortical, intermediate and medullary cell types) can be described in detail and the microanatomical relation of T cell subsets with other cell types studied.

已引入三种新技术用于人类胸腺细胞和T细胞的表型分析。不同亚类的单克隆抗体（即IgG1和IgG2）以各种组合使用，并用与不同荧光染料偶联的亚类特异性第二层进行标记。用单克隆和传统试剂的组合研究组织切片，并使用抗小鼠Ig包被的大珠柱分离抗体标记的细胞。借助这些简单技术，可以详细描述胸腺细胞分化的四个阶段（前胸腺细胞、皮质、中间和髓质细胞类型），并研究T细胞亚群与其他细胞类型的显微解剖关系。