Phaosawasdi K, Fisher R S
Am J Physiol. 1982 Nov;243(5):G330-5. doi: 10.1152/ajpgi.1982.243.5.G330.
The control mechanisms of pyloric pressure responses have not been elucidated clearly. The purposes of this study were twofold: 1) to determine the dose-related pressure responses of the pylorus to exogenous glucagon, secretin, and cholecystokinin, and 2) to correlate changes in pyloric pressure with serum concentrations of these hormones. Pyloric pressures were measured by infusion manometry, and the serum concentrations of glucagon and secretin were quantitated using radioimmunoassays. Each of the hormones tested, glucagon, secretin, and cholecystokinin, increased the pyloric pressure significantly. The lowest active dosages tested for each of these peptide hormones were 2 micrograms.kg-1.h-1, 2 U.kg-1.h-1, and 1 U.kg-1.h-1, respectively. The maximal pyloric pressure responses recorded were 8.7 +/- 1.1 (P less than 0.05), 12.6 +/- 2.1 (P less than 0.02), and 14.8 +/- 1.7 (P less than 0.02) mmHg, respectively. The pyloric pressure responses to insulin hypoglycemia, duodenal acidification, and intraduodenal olive oil were 11.3 +/- 1.5, 13.4 +/- 1.4, and 11.3 +/- 1.4 mmHg, respectively. The serum concentrations of immunoreactive glucagon during infusion of the lowest active dosage of glucagon and insulin hypoglycemia were 801 +/- 55 and 322 +/- 12 pg/ml, respectively. The serum concentrations of immunoreactive secretin during infusion of the lowest active dosage of secretin and during duodenal acidification were 980 +/- 60 and 110 +/- 7.0 pg/ml, respectively. Although pyloric contraction can be induced by administration of exogenous glucagon, secretin, and cholecystokinin, these studies suggest that these effects may have no physiological relevance.
幽门压力反应的控制机制尚未完全阐明。本研究有两个目的：1）确定幽门对外源性胰高血糖素、促胰液素和胆囊收缩素的剂量相关压力反应；2）将幽门压力变化与这些激素的血清浓度相关联。通过灌注测压法测量幽门压力，使用放射免疫分析法对胰高血糖素和促胰液素的血清浓度进行定量。所测试的每种激素，即胰高血糖素、促胰液素和胆囊收缩素，均显著增加幽门压力。这些肽类激素各自测试的最低有效剂量分别为2微克·千克⁻¹·小时⁻¹、2单位·千克⁻¹·小时⁻¹和1单位·千克⁻¹·小时⁻¹。记录到的最大幽门压力反应分别为8.7±1.1（P<0.05）、12.6±2.1（P<0.02）和14.8±1.7（P<0.02）mmHg。幽门对胰岛素低血糖、十二指肠酸化和十二指肠内注入橄榄油的压力反应分别为11.3±1.5、13.4±1.4和11.3±1.4 mmHg。在输注最低有效剂量的胰高血糖素和胰岛素低血糖期间，免疫反应性胰高血糖素的血清浓度分别为801±55和322±12 pg/ml。在输注最低有效剂量的促胰液素期间和十二指肠酸化期间，免疫反应性促胰液素的血清浓度分别为980±60和110±7.0 pg/ml。虽然外源性胰高血糖素、促胰液素和胆囊收缩素的给药可诱导幽门收缩，但这些研究表明这些作用可能没有生理相关性。
