Lee J H
Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi. 1982 Aug;15(3):245-9.
The theophylline, phenobarbital and dilantin were measured in serum by rate nephelometric immunoassay with the Beckman Immunochemistry System (ICS, Beckman Inc. Brea., CA 92621). The procedures required less than three minutes after proper calibration and dilution, and only 42 microliters of serum theophylline and 100 microliters of serum for phenobarbital and dilantin per determination. It is simple and needs only pipetting and dilution with automatic dispenser and diluter. Day to day variation was determined by analyzing on 30 days two control sera containing theophylline level I (12 micrograms/ml 18-micrograms/ml) and level II (30 micrograms-38 micrograms/ml). The average theophylline level I concentration was 14.5 micrograms/ml, with a standard deviation of 0.70 micrograms/ml, and a coefficient of variation of 4.8%. The average theophylline level II concentration was 32.2 micrograms/ml, with a standard deviation of 1.89 micrograms/ml, and a coefficient of variation of 5.9%. Results attained in patients were compared with results of enzyme immunoassay procedure (EMIT, Syva Corp.). For theophylline, r-0.957, for phenobarbital, r=0.936, and for dilantin, r=0.972.
采用贝克曼免疫化学系统（ICS，贝克曼公司，加利福尼亚州布雷亚市92621）的速率散射比浊免疫分析法测定血清中的茶碱、苯巴比妥和苯妥英。经过适当校准和稀释后，该检测程序所需时间不到三分钟，每次测定仅需42微升血清茶碱以及100微升血清用于检测苯巴比妥和苯妥英。该方法简单，仅需使用自动移液器和稀释器进行移液和稀释操作。通过对两份含有I级（12微克/毫升至18微克/毫升）和II级（30微克至38微克/毫升）茶碱水平的对照血清进行30天的分析来确定日间变化。I级茶碱平均浓度为14.5微克/毫升，标准差为0.70微克/毫升，变异系数为4.8%。II级茶碱平均浓度为32.2微克/毫升，标准差为1.89微克/毫升，变异系数为5.9%。将患者的检测结果与酶免疫分析程序（EMIT，希瓦公司）的结果进行比较。茶碱的r值为0.957，苯巴比妥的r值为0.936，苯妥英的r值为0.972。
