Spooner B S, Holladay C R
Cell Motil. 1981;1(2):167-78. doi: 10.1002/cm.970010202.
Embryonic chick nerve cells, from dissociated dorsal root ganglia, were cultured on polylysine substrata and examined for tubulin and actin distribution by indirect immunofluorescence. Antibodies generated against chick brain tubulin produced specific fluorescence in growth cones, neurites, and cell bodies without revealing distribution differences or substructure in the nerve cells. However, at reduced antitubulin concentrations, differences were resolved. Tubulin fluorescence remained uniform and intense in neurites and cell bodies, but exhibited reduced intensity and patterning in growth cones. Nonneuronal cells in the cultures served as controls for typical cytoplasmic tubulin fluorescence distribution. Straining controls demonstrated that fluorescence resulted from tubulin-antitubulin binding. Analogous studies, using antibodies generated against chick brain actin, demonstrated distribution differences at reduced antiactin concentrations, including "hot spots" of intense fluorescence in growth cones and a paucity of fluorescence in neurites.
将来自解离背根神经节的鸡胚神经细胞培养在聚赖氨酸基质上，并通过间接免疫荧光检查微管蛋白和肌动蛋白的分布。针对鸡脑微管蛋白产生的抗体在生长锥、神经突和细胞体中产生特异性荧光，未显示神经细胞中的分布差异或亚结构。然而，在降低抗微管蛋白浓度时，差异得以显现。微管蛋白荧光在神经突和细胞体中保持均匀且强烈，但在生长锥中强度降低且呈现图案化。培养物中的非神经细胞作为典型细胞质微管蛋白荧光分布的对照。染色对照表明荧光是由微管蛋白 - 抗微管蛋白结合产生的。使用针对鸡脑肌动蛋白产生的抗体进行的类似研究表明，在降低抗肌动蛋白浓度时存在分布差异，包括生长锥中强烈荧光的“热点”以及神经突中荧光的缺乏。
