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肌动蛋白在大鼠脊髓神经节神经元胞体中的超微结构定位

Ultrastructural localization of actin in the cell body of rat spinal ganglion neurons.

作者信息

Pannese E, Procacci P, Ledda M

机构信息

Institute of Histology, Embryology and Neurocytology, University of Milan, Italy.

出版信息

Anat Embryol (Berl). 1996 Dec;194(6):527-31. doi: 10.1007/BF00187466.

DOI:10.1007/BF00187466
PMID:8957529
Abstract

We used phalloidin staining and immunocytochemistry at the light and electron microscope level to determine the localization of actin in the cell bodies of rat spinal ganglion neurons. The results show that actin is mostly concentrated along the periphery of the neuronal perikaryon, including the perikaryal projections. This localization places actin in a strategic position to be influenced by incoming signals and to produce mechanical tensions able to shape the perikaryal surface.

摘要

我们在光学显微镜和电子显微镜水平上使用鬼笔环肽染色和免疫细胞化学方法来确定肌动蛋白在大鼠脊髓神经节神经元细胞体中的定位。结果表明,肌动蛋白主要集中在神经元胞体的周边,包括胞体突起。这种定位使肌动蛋白处于一个关键位置,能够受到传入信号的影响,并产生能够塑造胞体表面的机械张力。

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本文引用的文献

1
On the influence of the perineuronal microenvironment on the outgrowth of perikaryal projections of spinal ganglion neurons.论神经周微环境对脊髓神经节神经元胞体投射生长的影响。
J Submicrosc Cytol Pathol. 1995 Jul;27(3):303-8.
2
Perikaryal projections of spinal ganglion neurons: quantitative differences between membrane domains in contact with different microenvironments.脊髓神经节神经元的胞体投射:与不同微环境接触的膜结构域之间的数量差异。
J Anat. 1994 Dec;185 ( Pt 3)(Pt 3):497-502.
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Development of microspikes and neurites in cultured dorsal root ganglion cells.
Neurosci Lett. 1982 May 17;30(1):1-5. doi: 10.1016/0304-3940(82)90002-7.
4
Immunocytochemical evidence for colocalization in neurite growth cones of actin and myosin and their relationship to cell--substratum adhesions.肌动蛋白和肌球蛋白在神经突生长锥中共定位的免疫细胞化学证据及其与细胞-基质黏附的关系。
Dev Biol. 1981 Jul 15;85(1):113-22. doi: 10.1016/0012-1606(81)90240-2.
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A quantitative electron microscope study of the perikaryal projections of sensory ganglion neurons. I. Cat and rabbit.感觉神经节神经元胞周突起的定量电子显微镜研究。I. 猫和兔。
J Comp Neurol. 1983 Mar 1;214(3):239-50. doi: 10.1002/cne.902140302.
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Arrangement of neurofilaments, microtubules and microfilament-associated proteins in cultured dorsal root ganglia cells.培养的背根神经节细胞中神经丝、微管和微丝相关蛋白的排列
Eur J Cell Biol. 1981 Apr;24(1):20-7.
7
Distribution of tubulin and actin in neurites and growth cones of differentiating nerve cells.微管蛋白和肌动蛋白在分化神经细胞的神经突和生长锥中的分布。
Cell Motil. 1981;1(2):167-78. doi: 10.1002/cm.970010202.
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Differences in the organization of actin in the growth cones compared with the neurites of cultured neurons from chick embryos.与鸡胚培养神经元的神经突相比,生长锥中肌动蛋白组织的差异。
J Cell Biol. 1983 Oct;97(4):963-73. doi: 10.1083/jcb.97.4.963.
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A quantitative electron microscope study of the perikaryal projections of sensory ganglion neurons. II. Gecko and lizard.感觉神经节神经元胞周投射的定量电子显微镜研究。II. 壁虎和蜥蜴。
J Comp Neurol. 1985 Oct 8;240(2):212-8. doi: 10.1002/cne.902400210.
10
Immunoelectron microscopic localization of actin in neurites of cultured embryonic chick dorsal root ganglia: actin is a component of granular, microtubule-associated crossbridges.胚胎鸡背根神经节培养神经元突起中肌动蛋白的免疫电子显微镜定位:肌动蛋白是颗粒状、与微管相关的交叉桥的组成成分。
Brain Res. 1988 Dec 6;474(2):279-86. doi: 10.1016/0006-8993(88)90441-6.