Auger C, Monier J C, Dardenne M, Pleau J M, Bach J F
Immunol Lett. 1982 Oct;5(4):213-6. doi: 10.1016/0165-2478(82)90137-7.
Using a new immunoelectromicroscopical technique, this study confirms the localization of the 'facteur thymique sérique' (FTS) in the reticulo-epithelial cells of mouse thymus. The use of anti-FTS monoclonal antibodies on ultrathin sections for electron microscopy reveals FTS in cytoplasmic vacuoles, labelling density depending on the density of the vacuolar content. The successful application of this technique opens the way to its use for double-immunolabelling.
本研究采用一种新的免疫电镜技术，证实了“血清胸腺因子”（FTS）在小鼠胸腺网状上皮细胞中的定位。在超薄切片上使用抗FTS单克隆抗体进行电子显微镜观察，发现FTS存在于细胞质空泡中，标记密度取决于空泡内容物的密度。该技术的成功应用为其用于双重免疫标记开辟了道路。
