Takahashi A, Shimazu T
J Auton Nerv Syst. 1982 Sep;6(2):225-35. doi: 10.1016/0165-1838(82)90053-4.
The rates of fatty acid synthesis and syntheses of other lipids were measured in vivo by determining 3H-incorporation from tritiated water or 14C-incorporation from [U-14C]glucose into white adipose tissues (WAT), brown adipose tissue (BAT) and liver, during electrical stimulation of the ventromedial hypothalamic nucleus (VMH) and the lateral hypothalamic nucleus (LH) of female rats. Electrical stimulation of the VMH markedly increased the rate of fatty acid synthesis and the conversion of glucose to total lipids, glyceride glycerol and phospholipids in interscapular BAT, but not in parametrial or retroperitoneal WAT. Conversely, on VMH stimulation, the syntheses of total lipids, glyceride glycerol and phospholipids from glucose in the liver decreased slightly, though not significantly. Electrical stimulation of the LH had no such effect. Administration of insulin increased the rate of fatty acid synthesis in both brown and white adipose tissues and in the liver. The rate of disappearance of [14C]glucose and the concentration of insulin in the blood were not changed significantly by stimulation of the VMH. These data indicate that electrical stimulation of the VMH, but not the LH, enhances lipogenesis in BAT preferentially through a mechanism not involving insulin, but probably through activation of sympathetic innervation of BAT. The physiological significance of the findings is discussed in relation to hypothalamic function in controlling non-shivering thermogenesis and obesity.
通过测定氚化水的3H掺入或[U-14C]葡萄糖的14C掺入到白色脂肪组织（WAT）、棕色脂肪组织（BAT）和肝脏中的情况，在电刺激雌性大鼠腹内侧下丘脑核（VMH）和外侧下丘脑核（LH）期间，体内测量脂肪酸合成及其他脂质的合成速率。电刺激VMH显著增加肩胛间BAT中脂肪酸合成速率以及葡萄糖向总脂质、甘油三酯甘油和磷脂的转化，但在子宫旁或腹膜后WAT中未出现这种情况。相反，在VMH刺激时，肝脏中由葡萄糖合成的总脂质、甘油三酯甘油和磷脂略有下降，尽管不显著。电刺激LH没有这种作用。给予胰岛素可增加棕色和白色脂肪组织以及肝脏中的脂肪酸合成速率。刺激VMH后，[14C]葡萄糖的消失速率和血液中胰岛素浓度没有显著变化。这些数据表明，电刺激VMH而非LH优先通过一种不涉及胰岛素的机制增强BAT中的脂肪生成，但可能是通过激活BAT的交感神经支配。结合下丘脑在控制非颤抖性产热和肥胖中的功能，讨论了这些发现的生理学意义。
