Azzolina L S, Fracastoro G, Pisarri N, Lievore R, Cordiano C, Tridente G
Tumori. 1978 Apr 30;64(2):151-60. doi: 10.1177/030089167806400205.
The sera of 212 patients with malignant and non-malignant diseases have been radioimmunoassayed for the presence of the carcinoembryonic antigen (CEA) using 3 different kits produced of Hoffman La Roche, Switzerland (RCK), BY Sorin-IRE, Italy and Belgium (SCK), and by the Istituto Sieroterapico Milanese, Italy (ICK). In the presence of endodermically-derived system carcinomas, the RCK gave more positive results (72.6%) than did the SCK (63.1%) or ICK (56.2%). With regard to other carcinomas, ICK (50.0%) and SCK (47.1%) gave better results than did RCK (30.6%). The results are discussed in terms of clinical usefulness of the CEA assay and as regards reproducibility, procedural advantages, and economical cost of each kit. It is concluded that the CEA assay cannot be used for the diagnosis of gastrointestinal cancers, although it is useful as a measure of "cancerosity" for prognostic purposes. In this sense the double antibody method employed by SCK and ICK is clinically more advantageous than is the perchloric acid extraction-zirconyl phosphate gel precipitation method of RCK.
采用瑞士霍夫曼·罗氏公司生产的3种不同试剂盒（RCK）、意大利和比利时索林 - IRE公司的试剂盒（SCK）以及意大利米兰血清治疗研究所的试剂盒（ICK），对212例患有恶性和非恶性疾病患者的血清进行了癌胚抗原（CEA）放射免疫分析。在内胚层来源的系统癌患者中，RCK检测出的阳性结果（72.6%）比SCK（63.1%）或ICK（56.2%）更多。对于其他癌症，ICK（50.0%）和SCK（47.1%）的检测结果比RCK（30.6%）更好。从CEA检测的临床实用性以及各试剂盒的可重复性、操作优势和经济成本等方面对结果进行了讨论。得出的结论是，CEA检测不能用于胃肠道癌症的诊断，尽管它作为评估“癌症程度”以用于预后目的是有用的。从这个意义上讲，SCK和ICK采用的双抗体法在临床上比RCK的高氯酸提取 - 磷酸锆凝胶沉淀法更具优势。
