Wagener C, Breuer H
J Clin Chem Clin Biochem. 1978 Jun;16(6):323-7. doi: 10.1515/cclm.1978.16.6.323.
Human carcinoembryonic antigen (CEA) was measured in perchloric acid extracts from 9 malignant gastrointestinal tumours, using two different radioimmunological methods (Hoffmann-La Roche and CEA-IRE-SORIN). When standard curves were set up, quantitative differences in the degree of inhibition were found between the standards from the two manufacturers. In the Hoffmann-La Roche assay the CEA binding curves of all tumour extracts ran parallel with the standard. However, when the CEA-IRE-SORIN assay was applied two of the nine tumours exhibited different slopes. In one case, using the CEA-IRE-SORIN method, various parts of the tumour exhibited binding curves with non-parallel slopes. The present results suggest that radioimmunologically heterogeneous CEA occurs in different gastrointestinal tumours as well as in various parts of the same tumour.
采用两种不同的放射免疫法（霍夫曼-罗氏法和CEA-IRE-索林法）对9例恶性胃肠道肿瘤的高氯酸提取物中的人癌胚抗原（CEA）进行了检测。在绘制标准曲线时，发现两家制造商的标准品在抑制程度上存在定量差异。在霍夫曼-罗氏检测中，所有肿瘤提取物的CEA结合曲线与标准曲线平行。然而，应用CEA-IRE-索林检测时，9例肿瘤中有2例呈现出不同的斜率。在1例中，使用CEA-IRE-索林法，肿瘤的不同部位呈现出斜率不平行的结合曲线。目前的结果表明，放射免疫异质性CEA存在于不同的胃肠道肿瘤以及同一肿瘤的不同部位。
