一种用于常规评估胃肠道癌患者循环癌胚抗原免疫复合物的方法。
An approach to the routine estimation of circulating carcinoembryonic antigen immune complexes in patients with carcinomata of the gastrointestinal tract.
作者信息
Kapsopoulou-Dominos K, Anderer F A
出版信息
Clin Exp Immunol. 1979 Jul;37(1):25-32.
Abstract
The carcinoembryonic antigen (CEA) distribution obtained after fractionation of thirty sera of patients with gastrointestinal tumours by gel filtration in Sephadex G200 was compared with that resulting from extraction of the sera with perchloric acid. In all cases gel filtration yielded a fraction of free CEA and in twenty-three cases an additional fraction of CEA immune complexes. The amounts of free CEA corresponded fairly well to the fractions of CEA which could be extracted by perchloric acid. The CEA content of the fraction containing CEA immune complexes was comparable to the CEA content of the perchloric acid precipitates. The presence of CEA immune complexes in the perchloric acid precipitates could be demonstrated by gel filtration under dissociating andnon-dissociating conditions and after pre-incubation with 125I-CEA by radioimmuno-double-diffusion using monospecific rabbit antisera against human IgM and IgG.
摘要
对30例胃肠道肿瘤患者的血清进行Sephadex G200凝胶过滤分级分离后获得的癌胚抗原（CEA）分布，与用高氯酸提取血清所得的结果进行了比较。在所有病例中，凝胶过滤均产生了一部分游离CEA，23例还产生了一部分CEA免疫复合物。游离CEA的量与可被高氯酸提取的CEA部分相当吻合。含CEA免疫复合物部分的CEA含量与高氯酸沉淀物的CEA含量相当。通过在解离和非解离条件下的凝胶过滤以及在与125I-CEA预温育后，使用针对人IgM和IgG的单特异性兔抗血清进行放射免疫双扩散，可以证明高氯酸沉淀物中存在CEA免疫复合物。
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DEMONSTRATION OF TUMOR-SPECIFIC ANTIGENS IN HUMAN COLONIC CARCINOMATA BY IMMUNOLOGICAL TOLERANCE AND ABSORPTION TECHNIQUES.通过免疫耐受和吸收技术证明人类结肠癌中的肿瘤特异性抗原
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Elution of "blocking factors" from human tumors, capable of abrogating tumor-cell destruction by specifically immune lymphocytes.从人类肿瘤中洗脱“封闭因子”，这些因子能够消除特异性免疫淋巴细胞对肿瘤细胞的破坏作用。
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