一种用于常规评估胃肠道癌患者循环癌胚抗原免疫复合物的方法。
An approach to the routine estimation of circulating carcinoembryonic antigen immune complexes in patients with carcinomata of the gastrointestinal tract.
作者信息
Kapsopoulou-Dominos K, Anderer F A
出版信息
Clin Exp Immunol. 1979 Jul;37(1):25-32.
Abstract
The carcinoembryonic antigen (CEA) distribution obtained after fractionation of thirty sera of patients with gastrointestinal tumours by gel filtration in Sephadex G200 was compared with that resulting from extraction of the sera with perchloric acid. In all cases gel filtration yielded a fraction of free CEA and in twenty-three cases an additional fraction of CEA immune complexes. The amounts of free CEA corresponded fairly well to the fractions of CEA which could be extracted by perchloric acid. The CEA content of the fraction containing CEA immune complexes was comparable to the CEA content of the perchloric acid precipitates. The presence of CEA immune complexes in the perchloric acid precipitates could be demonstrated by gel filtration under dissociating andnon-dissociating conditions and after pre-incubation with 125I-CEA by radioimmuno-double-diffusion using monospecific rabbit antisera against human IgM and IgG.
摘要
对30例胃肠道肿瘤患者的血清进行Sephadex G200凝胶过滤分级分离后获得的癌胚抗原（CEA）分布，与用高氯酸提取血清所得的结果进行了比较。在所有病例中，凝胶过滤均产生了一部分游离CEA，23例还产生了一部分CEA免疫复合物。游离CEA的量与可被高氯酸提取的CEA部分相当吻合。含CEA免疫复合物部分的CEA含量与高氯酸沉淀物的CEA含量相当。通过在解离和非解离条件下的凝胶过滤以及在与125I-CEA预温育后，使用针对人IgM和IgG的单特异性兔抗血清进行放射免疫双扩散，可以证明高氯酸沉淀物中存在CEA免疫复合物。
相似文献
1
An approach to the routine estimation of circulating carcinoembryonic antigen immune complexes in patients with carcinomata of the gastrointestinal tract.
Clin Exp Immunol. 1979 Jul;37(1):25-32.
2
Circulating carcinoembryonic antigen immune complexes in sera of patients with carcinomata of the gastrointestinal tract.
Clin Exp Immunol. 1979 Feb;35(2):190-5.
3
[Prognostic value of circulating immune complexes of carcinoembryonic antigen (CEA) in patients with adenocarcinoma of the gastrointestinal tract (author's transl)].
J Clin Chem Clin Biochem. 1980 Mar;18(3):175-81.
4
Localization of carcinoembryonic antigen in mesenteric lymph nodes of patients with gastrointestinal cancer.
Neoplasma. 1983;30(1):67-72.
5
[Isolation of carcinoembryonic antigen].
Acta Biol Med Ger. 1975;34(8):1347-57.
6
Preliminary immunologic characterization of glycoprotein antigens (CEA) of tumors of gastrointestinal tract in humans.
Arch Immunol Ther Exp (Warsz). 1975;23(2):247-55.
7
Isolation of anti-carcinoembryonic antigen (CEA), specific immunoglobulins and their use in immunocytochemical and enzyme-immunoassays (ELISA).
Arch Roum Pathol Exp Microbiol. 1989 Jul-Sep;48(3):227-35.
8
[The isolation of anti-carcinoembryonic antigen (CEA) immunoglobulins and their use in immunocytochemical and immunoenzyme (ELISA) technics].
Rev Ig Bacteriol Virusol Parazitol Epidemiol Pneumoftiziol Bacteriol Virusol Parazitol Epidemiol. 1989 Jul-Sep;34(3):233-40.
9
A comparison of methods for the isolation of carcinoembryonic antigen.
Cancer Res. 1978 Mar;38(3):619-23.
10
Are circulating CEA immune complexes a prognostic marker in patients with carcinoma of the gastrointestinal tract?
Br J Cancer. 1980 Jul;42(1):26-33. doi: 10.1038/bjc.1980.199.
引用本文的文献
1
Biological therapy: approaches in colorectal cancer. Strategies to enhance carcinoembryonic antigen (CEA) as an immunogenic target.
Br J Cancer. 1998 Mar;77(5):683-93. doi: 10.1038/bjc.1998.114.
2
The presence of anti-carcinoembryonic antigen (CEA) antibodies in the sera of patients with gastrointestinal malignancies.
J Clin Immunol. 1994 Sep;14(5):310-3. doi: 10.1007/BF01540984.
3
Immune response to the carcinoembryonic antigen in patients treated with an anti-idiotype antibody vaccine.
J Clin Invest. 1995 Jul;96(1):334-42. doi: 10.1172/JCI118039.
4
[Relapse prognosis for patients with adenocarcinoma of the gastrointestinal tract on the basis of carcinoembryonic antigen (CEA) and its circulating immune complexes (author's transl)].
Klin Wochenschr. 1980 Feb 1;58(3):125-33. doi: 10.1007/BF01477269.
5
Circulating immune complexes (CIC), carcinoembryonic antigen (CEA) and CIC containing CEA as markers for colorectal cancer.
Clin Exp Immunol. 1984 Dec;58(3):685-93.
6
Immune complexes in human diseases: a review.
Am J Pathol. 1980 Aug;100(2):529-94.
7
CEA-containing immune complexes in sera of patients with colorectal and breast cancer--analysis of complexed immunoglobulin classes.
Cancer Immunol Immunother. 1988;26(2):180-4. doi: 10.1007/BF00205613.
8
A rapid method for the determination of human CEA/mouse anti-CEA immune complexes in patients undergoing immunoscintigraphy.
Eur J Nucl Med. 1989;15(6):321-5. doi: 10.1007/BF00435473.
本文引用的文献
1
DEMONSTRATION OF TUMOR-SPECIFIC ANTIGENS IN HUMAN COLONIC CARCINOMATA BY IMMUNOLOGICAL TOLERANCE AND ABSORPTION TECHNIQUES.
J Exp Med. 1965 Mar 1;121(3):439-62. doi: 10.1084/jem.121.3.439.
2
Suggestive evidence that the "blocking antibodies" of tumor-bearing individuals may be antigen--antibody complexes.
Proc Natl Acad Sci U S A. 1971 Jun;68(6):1372-5. doi: 10.1073/pnas.68.6.1372.
3
Demonstration of an antigen common to several varieties of neoplasia.
N Engl J Med. 1971 Jul 15;285(3):138-41. doi: 10.1056/NEJM197107152850302.
4
Blocking of cell-mediated tumor immunity by sera from patients with growing neoplasms.
Int J Cancer. 1971 Mar 15;7(2):226-37. doi: 10.1002/ijc.2910070206.
5
Elution of "blocking factors" from human tumors, capable of abrogating tumor-cell destruction by specifically immune lymphocytes.
Int J Cancer. 1972 Mar 15;9(2):274-83. doi: 10.1002/ijc.2910090205.
6
Role of serial plasma C.E.A. assays in detection of recurrent and metastatic colorectal carcinomas.
Br Med J. 1974 Nov 16;4(5941):382-5. doi: 10.1136/bmj.4.5941.382.
7
Usefulness of serial serum carcinoembryonic antigen (CEA) determinations during anticancer therapy or long-term follow-up of gastrointestinal carcinoma.
Cancer. 1974 Oct;34(4):1230-4. doi: 10.1002/1097-0142(197410)34:4<1230::aid-cncr2820340432>3.0.co;2-1.
8
Brief communication: carcinoembryonic antigen in colon cancer: absence in perchloric acid precipitates of plasma.
J Natl Cancer Inst. 1973 Sep;51(3):1081-3. doi: 10.1093/jnci/51.3.1081.
9
Blocking of lymphocyte-mediated cytotoxicity for rat hepatoma cells by tumour-specific antigen-antibody complexes.
Nat New Biol. 1972 Aug 9;238(84):185-6. doi: 10.1038/newbio238185a0.
10
Detection of recurrence of large-bowel carcinoma by radioimmunoassay of circulating carcinoembryonic antigen (C.E.A.).
Lancet. 1974 Sep 7;2(7880):535-40. doi: 10.1016/s0140-6736(74)91872-8.
Zotero 插件