Barnett S B, Miller M W, Cox C, Carstensen E L
Ultrasonics Institute, Sydney, Australia.
Ultrasound Med Biol. 1988;14(5):397-403. doi: 10.1016/0301-5629(88)90075-0.
This study comprised two repeat experiments in which Chinese hamster ovary (CHO) cells were exposed to nominal peak intensity Im = 2500 W/cm2, 2.5 MHz, pulsed ultrasound. Cells contained in a 0.53 cm diameter chamber in 0.48 cm thick Plexiglas were exposed to 10 microseconds pulses at 200 Hz prf in 9 sites for 3 min per site for a total duration of 27 min. The -3 dB focal beamwidth was 0.115 cm at an axial distance of 4.8 cm. Analysis of the pooled data from each series of experiments showed a statistically significant increase in the mean frequency of sister chromatid exchanges (SCE) in exposed compared to control cells. The results show a high degree of variability in the controls and insonated samples, which suggests that large numbers of insonations may be required in studies with SCE to reduce the high probability of missing an effect. The results of this series of experiments provide the first confirmation of an increase in SCEs following exposure to pulsed ultrasound.
本研究包含两个重复实验，其中将中国仓鼠卵巢（CHO）细胞暴露于标称峰值强度Im = 2500 W/cm²、2.5 MHz的脉冲超声下。置于0.48 cm厚有机玻璃制成的直径0.53 cm的腔室中的细胞，在9个位点以200 Hz脉冲重复频率接受10微秒的脉冲照射，每个位点照射3分钟，总时长27分钟。在轴向距离4.8 cm处，-3 dB焦点束宽为0.115 cm。对每个实验系列的汇总数据进行分析后发现，与对照细胞相比，暴露细胞中姐妹染色单体交换（SCE）的平均频率有统计学上的显著增加。结果显示，对照和受声样本存在高度变异性，这表明在SCE研究中可能需要大量的照射次数，以降低错过效应的高概率。这一系列实验的结果首次证实了暴露于脉冲超声后SCE会增加。
