Jaros P P, Gäde G
Cell Tissue Res. 1982;227(3):555-62. doi: 10.1007/BF00204785.
Histological sections of the brain, suboesophageal ganglion, and the corpora cardiaca/corpora allata complex were examined for the presence of crustacean hyperglycemic hormone-like immunoreactive material. With the use of an antiserum directed against the hyperglycemic hormone of Carcinus maenas, immunofluorescence was found in the median portion of the pars intercerebralis, and the corpora cardiaca. Extracts of corpora cardiaca were examined by radioimmunoassay for competitive binding to the antiserum; one pair of corpora cardiaca contains at least 7 pg crustacean hyperglycemic hormone-like material.
对脑、咽下神经节以及心侧体/咽侧体复合体的组织切片进行检查,以确定是否存在类甲壳动物高血糖激素免疫反应性物质。使用针对食蟹猴高血糖激素的抗血清,在脑间部的中间部分和心侧体中发现了免疫荧光。通过放射免疫测定法检测心侧体提取物与抗血清的竞争性结合;一对心侧体至少含有7皮克类甲壳动物高血糖激素物质。