Clottens F, Gäde G, Huybrechts R, De Loof A
Zoological Institute, K. U. Leuven, Belgium.
Cell Tissue Res. 1989 Dec;258(3):631-6. doi: 10.1007/BF00218876.
A polyclonal antiserum was prepared against an N-terminal modified Cam-HrTH-II (Leu-Asn-Phe-...), one of the members of the large AKH/RPCH peptide family, first isolated from Carausius morosus. The localisation of this peptide was performed by means of immunocytochemical methods in the brain and corpora cardiaca-corpora allata complex of the stick insect, Carausius morosus and the grey fleshfly, Sarcophaga bullata. The distribution patterns of molecules reactive to the Cam-HrTH-II and the Lom-AKH-I antisera in both insect species were compared. In Carausius, both antisera reacted in the same cell bodies. In Sarcophaga, some neurons were stained by both, others only by one of the two antisera. By combining two different antisera, we demonstrated that there are no Lom-AKH-I-like molecules present in Carausius and that there must occur at least three different AKH-like molecules in the brain of Sarcophaga. One is similar to Cam-HrTH-II, the second to Lom-AKH-I and the third is an AKH/RPCH-like peptide, different from Lom-AKH-I and Cam-HrTH-II.
针对一种N端修饰的Cam-HrTH-II（亮氨酸-天冬酰胺-苯丙氨酸……）制备了多克隆抗血清，Cam-HrTH-II是从桑螵蛸首次分离出的大型AKH/RPCH肽家族成员之一。通过免疫细胞化学方法在竹节虫桑螵蛸以及麻蝇肉蝇的大脑和心侧体-咽侧体复合体中对该肽进行定位。比较了两种昆虫中与Cam-HrTH-II和Lom-AKH-I抗血清反应的分子的分布模式。在桑螵蛸中，两种抗血清在相同的细胞体中发生反应。在肉蝇中，一些神经元被两种抗血清都染色，另一些仅被两种抗血清中的一种染色。通过结合两种不同的抗血清，我们证明桑螵蛸中不存在Lom-AKH-I样分子，并且肉蝇大脑中至少存在三种不同的AKH样分子。一种与Cam-HrTH-II相似，第二种与Lom-AKH-I相似，第三种是一种AKH/RPCH样肽，与Lom-AKH-I和Cam-HrTH-II不同。
