Schmidt N J, Ota M, Gallo D, Fox V L
J Clin Microbiol. 1982 Oct;16(4):763-5. doi: 10.1128/jcm.16.4.763-765.1982.
Monoclonal antibodies conjugated with fluorescein permitted rapid, strain-specific identification of influenza A isolates and type-specific identification of influenza B isolates by direct immunofluorescence staining. Identification of H1 influenza A strains could be accomplished by direct immunofluorescence on cell culture fluids lacking sufficient hemagglutinin activity to permit identification by hemagglutination inhibition.
与荧光素结合的单克隆抗体通过直接免疫荧光染色,能够快速、菌株特异性地鉴定甲型流感病毒分离株,并能进行乙型流感病毒分离株的型特异性鉴定。对于血凝素活性不足以通过血凝抑制试验进行鉴定的细胞培养液,可通过直接免疫荧光法来鉴定H1甲型流感病毒株。