gamma-D-glutamylglycine as an antagonist of kainic acid on leech Retzius neurones.

Intracellular recordings were made from Retzius cells from the segmental ganglia of the leech, Hirudo medicinalis, gamma-D-Glutamylglycine, (50 microM), reversibly and preferentially antagonised the excitatory action of kainate, having no effect on actions of quisqualate or carbachol but at this concentration the antagonism was of short duration, reversing prior to washing off the gamma-D-glutamylglycine. However, higher concentrations of gamma-D-glutamylglycine (250-500 microM) reversibly antagonised both quisqualate and carbachol-induced excitatory responses, indicating a lack of specificity. The value of gamma-D-glutamylglycine in determining the possible site of action of kainate on leech Retzius cells is discussed.