Nørgaard-Pedersen B, Jensen S, Wandrup J
Prenat Diagn. 1982 Oct;2(4):257-64. doi: 10.1002/pd.1970020404.
A method for immunological detection of acetylcholinesterase (AChE) and cholinesterase (ChE) in amniotic fluid is described. By addition of a small amount of antihuman-erythrocyte membrane antibody or anti-pseudocholinesterase antibody to the sample before electrophoresis the two esterase bands on polyacrylamide gel (PAG) can be absorbed away. Similar staining results can also be obtained by specific inhibition of the two esterases with either BW 284C51 (AChE inhibitor) or Lysivane (ChE inhibitor). In cases with a faint AChE band and in cases with blood contamination the immune absorption technique makes interpretation easier. Nearly identical staining results have been obtained by the immune absorption technique and the inhibition technique in the following samples with an AChE band: 34 samples from pregnancies with severe fetal malformation or intrauterine death (2 cases), 4 fetal serum samples, 4 samples of cerebrospinal fluid, 4 samples of fetal erythrolysate and 4 samples of adult erythrolysate. It can be concluded that an antibody prepared against erythrocyte AChE cross-reacts with AChE in cerebrospinal fluid, and that this antibody can be used for demonstration of AChE in amniotic fluid.
本文描述了一种检测羊水乙酰胆碱酯酶（AChE）和胆碱酯酶（ChE）的免疫方法。在电泳前向样品中加入少量抗人红细胞膜抗体或抗假胆碱酯酶抗体，可将聚丙烯酰胺凝胶（PAG）上的两条酯酶带吸附掉。用BW 284C51（AChE抑制剂）或利多卡因（ChE抑制剂）特异性抑制这两种酯酶，也可得到类似的染色结果。对于AChE带较淡的情况以及有血液污染的情况，免疫吸附技术使结果判读更容易。通过免疫吸附技术和抑制技术，在以下含有AChE带的样本中获得了几乎相同的染色结果：34例严重胎儿畸形或宫内死亡妊娠（2例）的样本、4份胎儿血清样本、4份脑脊液样本、4份胎儿红细胞溶解产物样本和4份成人红细胞溶解产物样本。可以得出结论，抗红细胞AChE制备的抗体与脑脊液中的AChE发生交叉反应，并且该抗体可用于羊水AChE的检测。
