Massey T H, Barta J S
Ther Drug Monit. 1982;4(4):413-8. doi: 10.1097/00007691-198212000-00014.
We describe several modifications of the Syva EMIT assay for theophylline on the Instrumentation Laboratories Multistat III Micro Centrifugal Analyzer (MCA) designed to improve precision. These modifications include increased sample volume, increased time interval for obtaining the delta optical density, the use of buffer with no detergent to prevent the possibility of premix of reagents, and reversal of the order for loading the reagents. Furthermore, the loading of sample and reagents has been programmed to match the concentrations and sample volume/reagent volume ratios recommended by Syva. Precision analysis of the method yielded coefficient of variation values of less than 10% for controls (n = 52) and standards (n = 69). A cross-correlation study comparing the MCA method and the recommended method on the Gilford 3500 yielded a slope of 0.98, a y intercept of 0.07, and a correlation coefficient of 0.992. The method is precise, fast, and inexpensive, costing ¿63 per patient sample for a full rotor.
我们描述了为提高精密度而对Syva茶碱发射免疫分析方法在仪器实验室Multistat III微型离心机分析仪（MCA）上进行的几种改进。这些改进包括增加样本体积、增加获取吸光度差值的时间间隔、使用不含洗涤剂的缓冲液以防止试剂预混的可能性以及颠倒试剂加载顺序。此外，样本和试剂的加载已编程以匹配Syva推荐的浓度和样本体积/试剂体积比。该方法的精密度分析得出，对照品（n = 52）和标准品（n = 69）的变异系数值小于10%。一项比较MCA方法和吉尔福德3500上推荐方法的互相关研究得出，斜率为0.98，y轴截距为0.07，相关系数为0.992。该方法精确、快速且成本低廉，全转子每个患者样本的成本为63英镑。
