Zidwick M J, Rogers P
Biochem J. 1982 Dec 1;207(3):529-33. doi: 10.1042/bj2070529.
A highly sensitive radiochemical assay used to measure the synthesis and regulation of the product of the argH gene, argininosuccinase, in an Escherichia coli system in vitro is described. With L-[guanidino-14C]argininosuccinic acid as a substrate, and in the presence of excess arginase and urease, 14CO2 is collected in a simply designed micro-vessel. With this method less than 1 nmol of product can be measured in the presence of various concentrations of L-arginine.
本文描述了一种高灵敏度的放射化学分析方法，用于在体外大肠杆菌系统中测量argH基因产物精氨琥珀酸酶的合成与调控。以L-[胍基-¹⁴C]精氨琥珀酸为底物，在过量精氨酸酶和脲酶存在的情况下，¹⁴CO₂收集于一个设计简单的微血管中。用这种方法，在存在各种浓度L-精氨酸的情况下，可测量不到1 nmol的产物。
