携带大肠杆菌基因组不同区域的φ-80噬菌体转导颗粒的分离。
Isolation of transducing particles of phi-80 bacteriophage that carry different regions of the Escherichia coli genome.
作者信息
Press R, Glansdorff N, Miner P, De Vries J, Kadner R, Maas W K
出版信息
Proc Natl Acad Sci U S A. 1971 Apr;68(4):795-8. doi: 10.1073/pnas.68.4.795.
Abstract
It has been possible to mate two strains harboring F-prime (F') factors and to isolate from such matings rare recombinants that behave as though the two episomes had fused. Thus, two genes not previously linked may be brought into close proximity. An F' factor carrying the attachment site for varphi80 was fused with one carrying the met-ppc-arg region of the chromosome. Lysogenization of such a strain, followed by induction, led to the isolation of varphi80arg(+) and varphi80met(+) transducing phages. This technique may be utilized as a general method for joining diverse bacterial genes to the genome of phage varphi80.
摘要
已经能够使带有F-prime（F'）因子的两个菌株进行交配，并从这种交配中分离出罕见的重组体，这些重组体的行为就好像两个附加体已经融合。因此，两个先前未连锁的基因可能会紧密相邻。携带噬菌体φ80附着位点的F'因子与携带染色体met-ppc-arg区域的F'因子融合。对这样的菌株进行溶原化，然后诱导，导致分离出φ80arg（+）和φ80met（+）转导噬菌体。这种技术可以用作将不同细菌基因连接到噬菌体φ80基因组的通用方法。
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