[培养成纤维细胞有丝分裂过程中微管蛋白和α辅肌动蛋白的免疫组织化学观察]

[Immunohistochemical observations on tubulin and alpha actinin during mitosis of cultured fibroblasts].

作者信息

Arena N, Vergani G, Madeddu R, Satta G, Deiana L, Alia E E

出版信息

Boll Soc Ital Biol Sper. 1982 Dec 15;58(23):1552-7.

Abstract

In this work we studied alpha-actinin and tubulin sites in rabbit fibroblasts in culture. Antibodies anti-alpha-actinin were used for indirect PAP-reaction while antibodies anti-tubulin were used for indirect immunofluorescence method. The observations were carried out by light microscopy, phase-contrast and interference-contrast microscopy, with regard to actinin, and by fluorescence microscopy, with regard to tubulin. During the early mitotic phase, alpha-actinin is localized all over the cell membrane of the fibroblasts, forming a sort strong protective cap, while during diacinesis it forms only rings, localizing below the cell membrane and the philopodia. Thus tubulin forms the bundle fibres during mitotic phases.

摘要

在这项工作中，我们研究了培养的兔成纤维细胞中的α-辅肌动蛋白和微管蛋白位点。抗α-辅肌动蛋白抗体用于间接PAP反应，而抗微管蛋白抗体用于间接免疫荧光法。关于辅肌动蛋白，通过光学显微镜、相差显微镜和干涉对比显微镜进行观察；关于微管蛋白，则通过荧光显微镜进行观察。在有丝分裂早期，α-辅肌动蛋白定位于成纤维细胞的整个细胞膜上，形成一种强大的保护性帽，而在终变期，它仅形成环，定位于细胞膜和丝状伪足下方。因此，微管蛋白在有丝分裂期形成束状纤维。