有丝分裂过程中α-辅肌动蛋白在分裂沟中的定位。
Alpha-actinin localization in the cleavage furrow during cytokinesis.
作者信息
Fujiwara K, Porter M E, Pollard T D
出版信息
J Cell Biol. 1978 Oct;79(1):268-75. doi: 10.1083/jcb.79.1.268.
Abstract
We used antibodies against alpha-actinin and myosin labeled directly with contrasting fluorochromes to localize these contractile proteins simultaneously in dividing chick embryo cells. During mitosis anti-alpha-actinin stains diffusely the entire cytoplasm including the mitotic spindle, while in the same cells intense antimyosin staining delineates the spindle. During cytokinesis both antibodies stain the cleavage furrow intensely, and until the midbody forms the two staining patterns in the same cell are identical at the resolution of the light microscope. Thereafter the anti-alpha-actinin staining of the furrow remains strong, but the antimyosin staining diminishes. These observations suggest that alpha-actinin participates along with actin and myosin in the membrane movements associated with cytokinesis.
摘要
我们使用了分别直接标记有不同荧光染料的抗α-辅肌动蛋白抗体和抗肌球蛋白抗体，以便在分裂的鸡胚细胞中同时定位这些收缩蛋白。在有丝分裂期间，抗α-辅肌动蛋白弥漫性地染色整个细胞质，包括有丝分裂纺锤体，而在同一细胞中，强烈的抗肌球蛋白染色勾勒出纺锤体。在胞质分裂期间，两种抗体都强烈地染色分裂沟，并且直到中体形成之前，在光学显微镜的分辨率下，同一细胞中的两种染色模式是相同的。此后，分裂沟的抗α-辅肌动蛋白染色仍然很强，但抗肌球蛋白染色减弱。这些观察结果表明，α-辅肌动蛋白与肌动蛋白和肌球蛋白一起参与了与胞质分裂相关的膜运动。
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