一种盘基网柄菌的DNA片段可互补酿酒酵母ura3突变体。
A Dictyostelium discoideum DNA fragment complements a Saccharomyces cerevisiae ura3 mutant.
作者信息
Boy-Marcotte E, Jacquet M
出版信息
Gene. 1982 Dec;20(3):433-39. doi: 10.1016/0378-1119(82)90212-8.
PMID:6762322
Abstract
Dictyostelium discoideum DNA fragments have been inserted into the chimeric bacterium-yeast plasmid YEp13. Recombinant plasmids were used to transform yeast using a strain of Saccharomyces cerevisiae deficient in OMP decarboxylase activity. Several clones were selected for growth in uracil-free medium. One clone was further analysed and contains a plasmid with a segment of D. discoideum DNA which complements a yeast ura3 mutation.
摘要
盘基网柄菌的DNA片段已被插入到嵌合细菌-酵母质粒YEp13中。使用缺乏OMP脱羧酶活性的酿酒酵母菌株，用重组质粒转化酵母。选择了几个克隆在无尿嘧啶培养基中生长。对其中一个克隆进行了进一步分析，它含有一个带有盘基网柄菌DNA片段的质粒，该片段可互补酵母ura3突变。
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