Fiers W, Degrave W, Derynck R, Devos R, Gheysen D, Remaut E, Stanssens P, Tavernier J, Content J, De Clercq E
Princess Takamatsu Symp. 1982;12:227-36.
A clone has been identified which contains the genetic information specifying human fibroblast interferon (beta 1) and which was derived from a messenger RNA population. On the basis of the nucleotide sequence the complete amino acid sequence (166 residues) of human fibroblast interferon was deduced. It is preceded by a 21 amino acids long signal sequence. Also, a genomic clone was isolated and characterized in detail; on this basis we can conclude that the interferon beta 1 gene does not contain introns. The nucleotide sequence of the putative promoter region was deduced and compared with other eukaryotic promoters. So far, no evidence has been obtained for other human interferons of the fibroblast type. The interferon beta 1 was expressed in the mature form of Escherichia coli without glycosylation. Fully processed interferon was obtained by transfection of monkey CV1-cells with an SV40-derivative, in which the interferon gene replaced the major structural protein VP1.
已鉴定出一个克隆，它包含指定人成纤维细胞干扰素（β1）的遗传信息，并且来源于信使RNA群体。根据核苷酸序列推导了人成纤维细胞干扰素的完整氨基酸序列（166个残基）。它前面有一个21个氨基酸长的信号序列。此外，分离并详细鉴定了一个基因组克隆；据此我们可以得出结论，干扰素β1基因不包含内含子。推导了假定启动子区域的核苷酸序列并与其他真核启动子进行了比较。到目前为止，尚未获得关于其他成纤维细胞型人干扰素的证据。干扰素β1以未糖基化的成熟形式在大肠杆菌中表达。通过用SV40衍生物转染猴CV1细胞获得了完全加工的干扰素，其中干扰素基因取代了主要结构蛋白VP1。
