Nestmann E R, Stephen E R, Kowbel D J, Nasim A
Can J Genet Cytol. 1982;24(6):771-5. doi: 10.1139/g82-082.
A method is presented to screen chemicals for potential mutagenicity on the basis of their ability to cause more killing in cells of repair-deficient yeast than in wild type cells. Two species were chosen in the event that one might be more sensitive to certain chemicals. The strains used were RAD+ and rad6 derivatives of Saccharomyces cerevisiae and RAD+ and rad3 derivatives of Schizosaccharomyces pombe. This report describes the test system and results for 12 known, direct-acting mutagens (i.e., not requiring mammalian metabolic activation). These compounds showed more lethality in one or both of the repair-deficient strains, indicating that they induce damage to DNA which is subject to repair in wild type cells. Advantages of this system include the use of eukaryotic yeast cells which can be manipulated as easily as bacteria, and that exogenous enzymes (S9) can be added for metabolic activation. Growing yeast cells can activate certain promutagens, and preliminary experiments showed positive responses for diethylnitrosamine and 2-acetylaminofluorene without the addition of S9.
本文介绍了一种基于化学物质在修复缺陷型酵母细胞中比在野生型细胞中能导致更多细胞死亡的能力来筛选具有潜在致突变性化学物质的方法。选择了两个物种，以防其中一个对某些化学物质更敏感。所使用的菌株是酿酒酵母的RAD+和rad6衍生物以及粟酒裂殖酵母的RAD+和rad3衍生物。本报告描述了针对12种已知的直接作用诱变剂（即不需要哺乳动物代谢激活）的测试系统和结果。这些化合物在一种或两种修复缺陷型菌株中表现出更高的致死率，表明它们诱导了DNA损伤，而野生型细胞能够修复这种损伤。该系统的优点包括使用真核酵母细胞，其操作与细菌一样简便，并且可以添加外源酶（S9）进行代谢激活。生长中的酵母细胞可以激活某些前诱变剂，初步实验表明，在不添加S9的情况下，二乙基亚硝胺和2-乙酰氨基芴有阳性反应。
