Raveché E S, Steinberg A D
J Immunopharmacol. 1982;4(3):163-81. doi: 10.3109/08923978209026432.
The in vivo effects of two polyclonal immune stimulators were studied in several strains of mice by analyzing the percentages of cells in various phases of the cell cycle by flow cytometry. Both lipopolysaccharide (LPS) and polyriboinosinic X cytidylic acid (rI X rC) were capable of inducing an increase in the percentage of cells undergoing DNA synthesis (S phase) in the spleens of several mouse strains. The response to both LPS and rI X rC was maximal between days 3 and 5 following injection. Optimal in vivo responses to LPS occurred at 3-30 micrograms, and to rI X rC at 100 micrograms; however, responses were observed over a broad dose range. No similar increase in S-phase cells was observed following injection of non-mitogenic T-independent antigens. Specific antibody was also measured after in vivo administration of rI X rC. There was a dissociation between the ability of an injection to induce specific antibody and to induce proliferation. These studies extend our knowledge of in vivo lymphocyte activation, and provide a basis for a detailed analysis of lymphocyte activation following a variety of immune modulators in vivo.
通过流式细胞术分析细胞周期各阶段的细胞百分比，研究了两种多克隆免疫刺激剂在几种品系小鼠中的体内效应。脂多糖（LPS）和聚肌苷酸X胞苷酸（rI X rC）均能诱导几种小鼠品系脾脏中进行DNA合成（S期）的细胞百分比增加。注射后第3天至第5天对LPS和rI X rC的反应最大。对LPS的最佳体内反应发生在3 - 30微克，对rI X rC的最佳体内反应发生在100微克；然而，在很宽的剂量范围内都观察到了反应。注射非促有丝分裂的非T细胞依赖性抗原后，未观察到S期细胞有类似增加。在体内给予rI X rC后也检测了特异性抗体。注射诱导特异性抗体的能力与诱导增殖的能力之间存在分离。这些研究扩展了我们对体内淋巴细胞活化的认识，并为详细分析体内多种免疫调节剂作用后淋巴细胞的活化提供了基础。
